B075968
EMS: Biomedical Sciences
University of Edinburgh
Honours Project
Reproductive Biology
2019-20
Video 1. NK cell induced GzB-mediated killing event 1.
Human NK92 cells were co-cultured with MDA-MB-231 cells stably expressing the dual reporter pGFP-N1:NES-Casp8-GFP-T2A-GrzB-mCherry were imaged over time by time lapse microscopy. Images have been background subtracted and adjusted for brightness and contrast using Zen 3.0. Scale 100 μm.
Video 2. NK cell induced GzB-mediated killing event 2.
Human NK92 cells were co-cultured with MDA-MB-231 cells stably expressing the dual reporter pGFP-N1:NES-Casp8-GFP-T2A-GrzB-mCherry were imaged over time by time lapse microscopy. Images have been background subtracted and adjusted for brightness and contrast using Zen 3.0. Scale 100 μm.
Video 3. Casp8-mediated killing event with TRAIL. MDA-MB-231 cells stably expressing the dual reporter pGFP-N1:NES-Casp8-GFP-T2A-GrzB-mCherry and human recombinant TRAIL were imaged over time by time lapse microscopy. Images have been background subtracted and adjusted for brightness and contrast using Zen 3.0. Scale 100 μm.
Video 4. Casp8-mediated killing event with TRAIL. MDA-MB-231 cells stably expressing the dual reporter pGFP-N1:NES-Casp8-GFP-T2A-GrzB-mCherry and human recombinant TRAIL were imaged over time by time lapse microscopy. Images have been background subtracted and adjusted for brightness and contrast using Zen 3.0. Scale 100 μm.
All copyright belongs to Dr Takanori Kitamura
Principal Investigator, Chancellor’s Fellow & MRC Career Development Fellow
The Queen’s Medical Research Institute
Edinburgh BioQuarter
47 Little France Crescent
Edinburgh
EH16 4TJ